Enzyme+Catalysis

=Dirty Dozen Lab 2 Enzyme Catalysis= media type="youtube" key="hs75JmgGW5Y?version=3" height="315" width="560" media type="youtube" key="J14JX0NviLk?version=3" height="315" width="420" [|Lab Bench Lab 2] = = =Investigation 13 Enzyme Catalysis=
 * 4 parts:
 * Spontaneous decomposition of hydrogen peroxide
 * Enzyme catalyzed breakdown of hydrogen peroxide
 * Effects of temperature on catalase activity
 * Effects of pH on catalase activity
 * Probeware
 * If ever there was a lab that was destined for probeware, this is it! Here's what you'll need (no kit required and no titration involved):
 * Oxygen gas sensor and the data collection device (i.e. GLX, Spark, LabQuest, etc.)
 * 1.5% Hydrogen peroxide
 * Activated yeast suspension (source of the catalase)
 * 30 minutes prior to the lab add 600 mL of water water (30-35 degrees C) to 4 packages of dry yeast. Add 4g of sucrose (table sugar) and mix well.
 * Ice
 * Hot plate or microwave to prepare boiling water
 * 1M HCl and 1M NaOH
 * Kits
 * If you don't have probeware, I do recommend buying a kit. Each of these kits comes with syringes, which are much easier for students to use than a burette (and you don't need to worry about clean up). I've tried each of these and really have no preference. They're all about the same as far as materials. Frey happens to be the cheapest in this case.
 * [|Frey Lab 2]
 * [|Carolina Biological Lab 2]
 * [|Ward's Lab 2]
 * [|Flinn Lab 2]
 * Without a kit
 * Hydrogen peroxide and yeast can be bought locally (the yeast suspension is cheaper than purchasing pure catalase). If you're planning to conduct the titration, you will need to purchase H2SO4 and KMnO4.
 * [|Carolina Lab 13]
 * [|Ward's Lab 13]
 * [[file:AP Bio #13- NEW TOC.pdf]]
 * [|Flinn Lab 13]